By Kendra P. Rumbaugh, Iqbal Ahmad
This e-book offers a survey of contemporary advances within the improvement of antibiofilm brokers for medical and environmental functions. the truth that microbes exist in established groups referred to as biofilms has slowly turn into accredited in the scientific neighborhood. We now be aware of that over eighty% of all infectious ailments are biofilm-related; even if, major demanding situations nonetheless lie in our skill to diagnose and deal with those super recalcitrant infections.
Written by means of specialists from world wide, this booklet bargains a useful source for doctors looking to deal with biofilm-related sickness, educational and researchers drawn to drug discovery and teachers who train classes on microbial pathogenesis and clinical microbiology.
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Extra info for Antibiofilm Agents: From Diagnosis to Treatment and Prevention
Harmony Books, New York, NY, 46 p Hansen WL, Beuving J, Bruggeman CA, Wolffs PF (2010) Molecular probes for diagnosis of clinically relevant bacterial infections in blood cultures. J Clin Microbiol 48:4432–4438. 00562-10 [pii] Hendricks KJ, Burd TA, Anglen JO, Simpson AW, Christensen GD, Gainor BJ (2001) Synergy between Staphylococcus aureus and Pseudomonas aeruginosa in a rat model of complex orthopaedic wounds. J Bone Joint Surg Am 83-A:855–861 28 R. B. Cox Howell-Jones RS, Wilson MJ, Hill KE, Howard AJ, Price PE, Thomas DW (2005) A review of the microbiology, antibiotic usage and resistance in chronic skin wounds.
1985; Sun et al. 2011; Oliveira et al. 2010; Srivastava et al. 2011; Raghavachari et al. 2003); however, their efficacy is variable. Baschong et al. (2001) studied the effect of three separate reagents on reducing autofluorescence of three different types of tissue. These authors found that no single reagent was able to decrease autofluorescence in all samples and that the selection of an appropriate autofluorescence quenching agent required a trial-and-error search process. The nonspecific binding of molecular probes in tissue samples is another problem that can occur during imaging of biofilms.
In addition, while bacterial cells can be differentiated from host cells and structures by means of morphology, imaging of EPS poses a challenge. In this chapter, certain aspects that influence biofilm imaging in tissues will be discussed including sample collection, fixation, embedding and sectioning, staining, and microscopy. Although electron microscopy can be used for highresolution analysis of tissue samples, this chapter focuses primarily on fluorescence microscopy. Specific examples from published studies of microorganisms and biofilms in tissue are used when possible.